INTRACRISTAL LOCALIZATION OF SUCCINIC DEHYDROGENASE ACTIVITY WITH A NEW OSMIUM-CONTAINING TETRA-TETRAZOLIUM SALT
نویسندگان
چکیده
منابع مشابه
The Use of Tetranitro-blue Tetrazolium for the Cytochemical Localization of Succinic Dehydrogenase
Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced ...
متن کاملSuccinate Dehydrogenase Activity Assay in situ with Blue Tetrazolium Salt in Crabtree-Positive Saccharomyces cerevisiae Strain
A spectrophotometric method for determining succinate dehydrogenase (SDH) activity assay in azide-sensitive yeast Saccharomyces cerevisiae has been developed. The permeabilization of yeast cells by 0.05 % digitonin permitted to study yeast enzymatic activity in situ. The reduction of blue tetrazolium salt (BT) to blue tetrazolium formazan (BTf) was conducted in the presence of phenazine methosu...
متن کاملAn automated colorimetric (tetrazolium salt) assay for serum lactate dehydrogenase.
The adaptation of a tetrazolium salt assay for serum lactate dehydrogenase to full automation is described. The tetrazolium salt, INT, (2-p-iodophenyl-3-p-nitrophenyl5-phenyl tetrazolium chloride) serves as the chromogenic indicator of the enzyme activity. Phenazine methosulfate serves as an intermediate electron carrier. Estimation requires only 5 mm. of incubation, and assays may be run at th...
متن کاملA colorimetric method for the estimation of succinic dehydrogenase activity.
Many methods for the measurement of succinic dehydrogenase activity have been proposed (l-20). These have varied from measurement of the entire system of electron transport with oxygen as the final acceptor (5, 21), to the determination of succinic dehydrogenase activity itself (6, 9, 22). The assay of succinic dehydrogenase alone was made possible by the finding that phenazine methosulfate can...
متن کاملdetection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts
in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1968
ISSN: 0022-1554,1551-5044
DOI: 10.1177/16.7.487